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验证数据展示

  • WB analysis using 67083-1-Ig (same clone as 67083-1-PBS)

    WB analysis using 67083-1-Ig (same clone as 67083-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67083-1-Ig (SLC1A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67083-1-Ig (same clone as 67083-1-PBS)

    IHC staining of mouse brain using 67083-1-Ig (same clone as 67083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EAAT2/SLC1A2 antibody included
  • IHC staining of rat brain using 67083-1-Ig (same clone as 67083-1-PBS)

    IHC staining of rat brain using 67083-1-Ig (same clone as 67083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EAAT2/SLC1A2 antibody included
  • IHC staining of rat brain using 67083-1-Ig (same clone as 67083-1-PBS)

    IHC staining of rat brain using 67083-1-Ig (same clone as 67083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EAAT2/SLC1A2 antibody included
  • IHC staining of mouse brain using 67083-1-Ig (same clone as 67083-1-PBS)

    IHC staining of mouse brain using 67083-1-Ig (same clone as 67083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EAAT2/SLC1A2 antibody included

EAAT2 Monoclonal antibody, PBS Only

EAAT2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67083-1-PBS

产品说明书

CloneNo. 1C8D10

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

EAAT2, GLT 1, GLT1, SLC1A2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EAAT2/SLC1A2 Ready-To-Use IHC Kit. EAAT2/SLC1A2 primary antibody included.

产品信息

67083-1-PBS targets EAAT2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 EAAT2 fusion protein Ag19816 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 1 (glial high affinity glutamate transporter), member 2
别名 EAAT2, GLT 1, GLT1, SLC1A2
计算分子量 574 aa, 62 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC132768
基因名称 EAAT2
Gene ID (NCBI) 6506
RRIDAB_2882391
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP43004
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EAAT2 (also known as GLT-1), is encoded by SLC1A2 and is a glutamate transporter which can clear the majority of extracellular glutamate in brain and prevent brain seizures.Three isoforms of EAAT2 exist due to the alternative splicing. In addition to the 65-70 kDa monomer form, 130-150 kDa dimer of EAAT2 can also be observed on SDS-PAGE.(PMID: 22114258)