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EAAT2 Monoclonal antibody, PBS Only

EAAT2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C8D10

Cat no : 67083-1-PBS

Print datasheet

Synonyms

EAAT2, GLT 1, GLT1, SLC1A2

验证数据展示

  • WB analysis using 67083-1-Ig (same clone as 67083-1-PBS)

    WB analysis using 67083-1-Ig (same clone as 67083-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67083-1-Ig (SLC1A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67083-1-Ig (same clone as 67083-1-PBS)

    IHC staining of mouse brain using 67083-1-Ig (same clone as 67083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EAAT2/SLC1A2 antibody included
  • IHC staining of rat brain using 67083-1-Ig (same clone as 67083-1-PBS)

    IHC staining of rat brain using 67083-1-Ig (same clone as 67083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EAAT2/SLC1A2 antibody included
  • IHC staining of rat brain using 67083-1-Ig (same clone as 67083-1-PBS)

    IHC staining of rat brain using 67083-1-Ig (same clone as 67083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EAAT2/SLC1A2 antibody included
  • IHC staining of mouse brain using 67083-1-Ig (same clone as 67083-1-PBS)

    IHC staining of mouse brain using 67083-1-Ig (same clone as 67083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EAAT2/SLC1A2 antibody included
Planning an IHC experiment? We recommend our IHCeasy EAAT2/SLC1A2 Ready-To-Use IHC Kit. EAAT2/SLC1A2 primary antibody included.

产品信息

67083-1-PBS targets EAAT2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen EAAT2 fusion protein Ag19816 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name solute carrier family 1 (glial high affinity glutamate transporter), member 2
Synonyms EAAT2, GLT 1, GLT1, SLC1A2
Calculated Molecular Weight 574 aa, 62 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC132768
Gene Symbol EAAT2
Gene ID (NCBI) 6506
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43004
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EAAT2 (also known as GLT-1), is encoded by SLC1A2 and is a glutamate transporter which can clear the majority of extracellular glutamate in brain and prevent brain seizures.Three isoforms of EAAT2 exist due to the alternative splicing. In addition to the 65-70 kDa monomer form, 130-150 kDa dimer of EAAT2 can also be observed on SDS-PAGE.(PMID: 22114258)