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EAPP Polyclonal antibody

EAPP Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16931-1-AP

Print datasheet

Synonyms

E2F-associated phosphoprotein, E2F associated phosphoprotein, C14orf11, BM-036, BM036

验证数据展示

  • WB analysis of HEK-293 using 16931-1-AP

    WB analysis of HEK-293 using 16931-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16931-1-AP (EAPP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16931-1-AP

    IF Staining of HeLa using 16931-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16931-1-AP (EAPP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16931-1-AP targets EAPP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen EAPP fusion protein Ag10403 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name E2F-associated phosphoprotein
Synonyms E2F-associated phosphoprotein, E2F associated phosphoprotein, C14orf11, BM-036, BM036
Calculated Molecular Weight 285 aa, 33 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC001245
Gene Symbol EAPP
Gene ID (NCBI) 55837
RRIDAB_2878334
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ56P03
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EAPP antibody 16931-1-APDownload protocol
IF protocol for EAPP antibody 16931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols