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EBF1 Polyclonal antibody

EBF1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14159-1-AP

Print datasheet

Synonyms

COE1, Early B cell factor, early B cell factor 1, EBF, EBF1, O/E 1, OE 1, OLF1, Transcription factor COE1

验证数据展示

  • WB analysis of U2OS using 14159-1-AP

    WB analysis of U2OS using 14159-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 14159-1-AP (EBF1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 14159-1-AP

    IHC staining of human stomach cancer using 14159-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14159-1-AP (EBF1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 14159-1-AP

    IHC staining of human stomach cancer using 14159-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14159-1-AP (EBF1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inU2OS cells, Raji cells, 3T3-L1 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14159-1-AP targets EBF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen EBF1 fusion protein Ag5341 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name early B-cell factor 1
Synonyms COE1, Early B cell factor, early B cell factor 1, EBF, EBF1, O/E 1, OE 1, OLF1, Transcription factor COE1
Calculated Molecular Weight 591 aa, 64 kDa
Observed Molecular Weight 57-67 kDa
GenBank Accession NumberBC038805
Gene Symbol EBF1
Gene ID (NCBI) 1879
RRIDAB_2231057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UH73
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EBF1, also named as O/E-1, OE-1, OLF1, COE1 and EBF, belongs to the COE family. It is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. EBF1 is a key factor for the specification of B-cell lineage.

实验方案

Product Specific Protocols
WB protocol for EBF1 antibody 14159-1-APDownload protocol
IHC protocol for EBF1 antibody 14159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article
mouseWB

iScience

Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9

Authors - Ting Wang
View Article
mouseWB

ACS Nano

Arginine Supplementation Targeting Tumor-Killing Immune Cells Reconstructs the Tumor Microenvironment and Enhances the Antitumor Immune Response

Authors - Junlei Zhang
View Article