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验证数据展示

  • WB analysis of U2OS using 14159-1-AP

    WB analysis of U2OS using 14159-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 14159-1-AP (EBF1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 14159-1-AP

    IHC staining of human stomach cancer using 14159-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14159-1-AP (EBF1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 14159-1-AP

    IHC staining of human stomach cancer using 14159-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14159-1-AP (EBF1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EBF1 Polyclonal antibody

EBF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14159-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

COE1, Early B cell factor, early B cell factor 1, EBF, EBF1, O/E 1, OE 1, OLF1, Transcription factor COE1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, Raji cells, 3T3-L1 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14159-1-AP targets EBF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 EBF1 fusion protein Ag5341 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 early B-cell factor 1
别名 COE1, Early B cell factor, early B cell factor 1, EBF, EBF1, O/E 1, OE 1, OLF1, Transcription factor COE1
计算分子量 591 aa, 64 kDa
观测分子量 57-67 kDa
GenBank蛋白编号BC038805
基因名称 EBF1
Gene ID (NCBI) 1879
RRIDAB_2231057
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UH73
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EBF1, also named as O/E-1, OE-1, OLF1, COE1 and EBF, belongs to the COE family. It is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. EBF1 is a key factor for the specification of B-cell lineage.

实验方案

Product Specific Protocols
WB protocol for EBF1 antibody 14159-1-APDownload protocol
IHC protocol for EBF1 antibody 14159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article
mouseWB

iScience

Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9

Authors - Ting Wang
View Article
mouseWB

ACS Nano

Arginine Supplementation Targeting Tumor-Killing Immune Cells Reconstructs the Tumor Microenvironment and Enhances the Antitumor Immune Response

Authors - Junlei Zhang
View Article