验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, pig spleen tissue |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human spleen tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12371-1-AP targets EBI3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human, mouse |
| 免疫原 | EBI3 fusion protein Ag3051 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Epstein-Barr virus induced 3 |
| 别名 | EBI3, EBV induced gene 3 protein, Epstein Barr virus induced 3, IL 27 subunit beta, IL 27B, IL27B, Interleukin 27 subunit beta |
| 计算分子量 | 229 aa, 25 kDa |
| 观测分子量 | 25-35 kDa |
| GenBank蛋白编号 | BC015364 |
| 基因名称 | EBI3 |
| Gene ID (NCBI) | 10148 |
| RRID | AB_10646470 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14213 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EBI3, a 34-kDa secreted glycoprotein, is a subunit in 2 distinct heterodimeric cytokines: IL-27 and IL-35. IL-27, composed of p28 (IL-27) and EBI3, can trigger signaling in T cells, B cells, and myeloid cells. IL-35, composed of EBI3 and the ubiquitously expressed p35 subunit of IL-12, is an inhibitory cytokine involved in regulatory T-cell function. EBI3 plays a role in regulating cell-mediated immune responses. (PMID: 8551575; 18033300; 17874423; 21931777)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EBI3 antibody 12371-1-AP | Download protocol |
| IHC protocol for EBI3 antibody 12371-1-AP | Download protocol |
| IP protocol for EBI3 antibody 12371-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS One Epstein-Barr virus-induced gene 3 commits human mesenchymal stem cells to differentiate into chondrocytes via endoplasmic reticulum stress sensor | ||
Pathog Dis Accumulation of EBI3 induced by virulent Mycobacterium tuberculosis inhibits apoptosis in murine macrophages.
|
