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EBI3 Monoclonal antibody, PBS Only

EBI3 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F3H10

Cat no : 66354-1-PBS

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Synonyms

EBI3, EBV induced gene 3 protein, Epstein Barr virus induced 3, IL 27 subunit beta, IL 27B, IL27B, Interleukin 27 subunit beta

验证数据展示

  • WB analysis of human spleen using 66354-1-Ig (same clone as 66354-1-PBS)

    WB analysis of human spleen using 66354-1-Ig (same clone as 66354-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66354-1-Ig (EBI3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66354-1-PBS in a different storage buffer formulation.

产品信息

66354-1-PBS targets EBI3 in WB, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, pig, rat
Immunogen EBI3 fusion protein Ag3051 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Epstein-Barr virus induced 3
Synonyms EBI3, EBV induced gene 3 protein, Epstein Barr virus induced 3, IL 27 subunit beta, IL 27B, IL27B, Interleukin 27 subunit beta
Calculated Molecular Weight 229 aa, 25 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC015364
Gene Symbol EBI3
Gene ID (NCBI) 10148
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14213
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EBI3, a 34-kDa secreted glycoprotein, is a subunit in 2 distinct heterodimeric cytokines: IL-27 and IL-35. IL-27, composed of p28 (IL-27) and EBI3, can trigger signaling in T cells, B cells, and myeloid cells. IL-35, composed of EBI3 and the ubiquitously expressed p35 subunit of IL-12, is an inhibitory cytokine involved in regulatory T-cell function. EBI3 plays a role in regulating cell-mediated immune responses. (PMID: 8551575; 18033300; 17874423; 21931777)