- Featured Product
- KD/KO Validated
ECD Polyclonal antibody
ECD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ECD Ready-To-Use IHC Kit. ECD primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
10192-1-AP targets ECD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | ECD fusion protein Ag0245 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ecdysoneless homolog (Drosophila) |
| Synonyms | Protein ecdysoneless homolog, Human suppressor of GCR two, hSGT1 |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | BC000721 |
| Gene Symbol | ECD |
| Gene ID (NCBI) | 11319 |
| RRID | AB_2188183 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95905 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The human suppressor of GCR two (hSGT1) was isolated by its ability to complement the growth defect of the gcr2 mutant of Saccharomyces cerevisiae. Further work confirmed that the rescue of growth was associated with recovery of glycolytic enzyme activities of gcr2, and that hSGT1 did not complement the growth defect of a gcr1 mutant. A hybrid protein comprising hSgt1p and the DNA-binding domain of Gal4p (GBD) activated a GAL1-lacZ reporter gene fusion, suggesting that the cloned gene may be a transcriptional activator. Two-hybrid experiments in yeast also indicate that hSgt1p interacts with Gcr1p. Northern analysis showed that hSGT1 is highly expressed in muscle and heart.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ECD antibody 10192-1-AP | Download protocol |
| IHC protocol for ECD antibody 10192-1-AP | Download protocol |
| IF protocol for ECD antibody 10192-1-AP | Download protocol |
| IP protocol for ECD antibody 10192-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget Amplification of ACK1 promotes gastric tumorigenesis via ECDdependent p53 ubiquitination degradation.
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