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ECHDC1 Polyclonal antibody

ECHDC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27732-1-AP

Print datasheet

Synonyms

ECHDC1

验证数据展示

  • WB analysis of PC-12 using 27732-1-AP

    WB analysis of PC-12 using 27732-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 27732-1-AP (ECHDC1 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IHC staining of mouse kidney using 27732-1-AP

    IHC staining of mouse kidney using 27732-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27732-1-AP (ECHDC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ECHDC1 antibody included
  • IHC staining of mouse kidney using 27732-1-AP

    IHC staining of mouse kidney using 27732-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27732-1-AP (ECHDC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ECHDC1 antibody included

经过测试的应用

Positive WB detected inPC-12 cells, A549 cells
Positive IHC detected inmouse kidney tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ECHDC1 Ready-To-Use IHC Kit. ECHDC1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27732-1-AP targets ECHDC1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ECHDC1 fusion protein Ag26905 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name enoyl Coenzyme A hydratase domain containing 1
Synonyms ECHDC1
Calculated Molecular Weight 307 aa, 34 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC003549
Gene Symbol ECHDC1
Gene ID (NCBI) 55862
RRIDAB_2880956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NTX5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ECHDC1 antibody 27732-1-APDownload protocol
IHC protocol for ECHDC1 antibody 27732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols