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ECHDC3 Polyclonal antibody

ECHDC3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27098-1-AP

Print datasheet

Synonyms

PP8332, PP1494, Enoyl-CoA hydratase domain-containing protein 3, mitochondrial

验证数据展示

  • WB analysis using 27098-1-AP

    WB analysis using 27098-1-AP

    MDCK lysates were subjected to SDS PAGE followed by western blot with 27098-1-AP (ECHDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 27098-1-AP

    IHC staining of human ovary cancer using 27098-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27098-1-AP (ECHDC3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMDCK cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27098-1-AP targets ECHDC3 in WB, IHC, ELISA applications and shows reactivity with human, canine samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, canine
Immunogen ECHDC3 fusion protein Ag25707 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name enoyl Coenzyme A hydratase domain containing 3
Synonyms PP8332, PP1494, Enoyl-CoA hydratase domain-containing protein 3, mitochondrial
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC009617
Gene Symbol ECHDC3
Gene ID (NCBI) 79746
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DC8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ECHDC3 antibody 27098-1-APDownload protocol
IHC protocol for ECHDC3 antibody 27098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols