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ECM1 Monoclonal antibody, PBS Only (Detector)

ECM1 Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6A12B3

Cat no : 66023-1-PBS

Print datasheet

Synonyms

ECM1, extracellular matrix protein 1, Secretory component p85

验证数据展示

  • WB analysis of MCF-7 using 66023-1-Ig (same clone as 66023-1-PBS)

    WB analysis of MCF-7 using 66023-1-Ig (same clone as 66023-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66023-1-Ig (same clone as 66023-1-PBS)

    IHC staining of human breast cancer using 66023-1-Ig (same clone as 66023-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ECM1 antibody included
  • IHC staining of human breast cancer using 66023-1-Ig (same clone as 66023-1-PBS)

    IHC staining of human breast cancer using 66023-1-Ig (same clone as 66023-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ECM1 antibody included
  • Sandwich ELISA standard curve of MP50074-1

    Sandwich ELISA standard curve of MP50074-1

    Sandwich ELISA standard curve of MP50074-1, ECM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68472-1-PBS. Detection antibody: 66023-1-PBS. Standard: Eg0533. Range: 0.156-10 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy ECM1 Ready-To-Use IHC Kit. ECM1 primary antibody included.

产品信息

66023-1-PBS targets ECM1 as part of a matched antibody pair:

MP50074-1: 68472-1-PBS capture and 66023-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ECM1 fusion protein Ag17955 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name extracellular matrix protein 1
Synonyms ECM1, extracellular matrix protein 1, Secretory component p85
Calculated Molecular Weight 540 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC023505
Gene Symbol ECM1
Gene ID (NCBI) 1893
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16610
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers.(21128013) It inhibits MMP9 proteolytic activity.