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ECM1 Recombinant antibody, PBS Only

ECM1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230321F12

Cat no : 83067-6-PBS

Print datasheet

Synonyms

Secretory component p85, extracellular matrix protein 1, 230321F12

验证数据展示

  • WB analysis using 83067-6-RR (same clone as 83067-6-PBS)

    WB analysis using 83067-6-RR (same clone as 83067-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83067-6-RR (ECM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83067-6-PBS in a different storage buffer formulation.

  • FC experiment of A375 using 83067-6-RR (same clone as 83067-6-PBS)

    FC experiment of A375 using 83067-6-RR (same clone as 83067-6-PBS)

    1x10^6 A375 cells were intracellularly stained with 0.25 ug Anti-Human ECM1 (83067-6-RR, Clone:230321F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83067-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83067-6-RR

    Affinity and Kinetic Characterization of 83067-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83067-6-RR against Human ECM1 were performed. The affinity constant is 0.419 nM.

Planning an IHC experiment? We recommend our IHCeasy ECM1 Ready-To-Use IHC Kit. ECM1 primary antibody included.

产品信息

83067-6-PBS targets ECM1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name extracellular matrix protein 1
Synonyms Secretory component p85, extracellular matrix protein 1, 230321F12
Calculated Molecular Weight 540 aa, 61 kDa
Observed Molecular Weight60-80 kDa
GenBank Accession NumberBC023505
Gene Symbol ECM1
Gene ID (NCBI) 1893
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16610
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ECM1 (extracellular matrix protein 1), also known as URBWD. It is located in secreted, extracellular space, and extracellular matrix, which is mainly expressed in esophagus and gall bladder. The gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. The calculated molecular weight of ECM1 is 60 kDa, and this antibody can recognize the 63 kDa isoform of target, moreover, this protein may have glycosylation modification.