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验证数据展示

  • IHC staining of mouse cerebellum using 21376-1-AP

    IHC staining of mouse cerebellum using 21376-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21376-1-AP (ECM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 21376-1-AP

    IHC staining of mouse cerebellum using 21376-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21376-1-AP (ECM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of L02 using 21376-1-AP

    IF Staining of L02 using 21376-1-AP

    Immunofluorescent analysis of L02 cells using 21376-1-AP (ECM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ECM2 Polyclonal antibody

ECM2 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 21376-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA and More (1)

别名

Matrix glycoprotein SC1/ECM2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse cerebellum tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21376-1-AP targets ECM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 ECM2 fusion protein Ag15913 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 extracellular matrix protein 2, female organ and adipocyte specific
别名 Matrix glycoprotein SC1/ECM2
计算分子量 699 aa, 80 kDa
GenBank蛋白编号BC107493
基因名称 ECM2
Gene ID (NCBI) 1842
RRIDAB_2878850
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94769
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for ECM2 antibody 21376-1-APDownload protocol
IF protocol for ECM2 antibody 21376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Microbiol Spectr

Transcriptome analysis reveals that PRV XJ delgE/gI/TK protects against intestinal damage in nose-dropping-infected mice by regulating ECM-ITGA/ITGB-P-FAK

Authors - Tong Xu
View Article
humanWB

Mol Neuropsychiatry

Cell Type-Specific Effects of Mutant DISC1: A Proteomics Study.

Authors - Meng Xia
View Article
humanIHC

Int J Biochem Cell Biol

ECM2, a prognostic biomarker for lower grade glioma, serves as a potential novel target for immunotherapy

Authors - Xingbo Cheng
View Article