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ECM2 Polyclonal antibody

ECM2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21376-1-AP

Print datasheet

Synonyms

Matrix glycoprotein SC1/ECM2

验证数据展示

  • IHC staining of mouse cerebellum using 21376-1-AP

    IHC staining of mouse cerebellum using 21376-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21376-1-AP (ECM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 21376-1-AP

    IHC staining of mouse cerebellum using 21376-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21376-1-AP (ECM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of L02 using 21376-1-AP

    IF Staining of L02 using 21376-1-AP

    Immunofluorescent analysis of L02 cells using 21376-1-AP (ECM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse cerebellum tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21376-1-AP targets ECM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ECM2 fusion protein Ag15913 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name extracellular matrix protein 2, female organ and adipocyte specific
Synonyms Matrix glycoprotein SC1/ECM2
Calculated Molecular Weight 699 aa, 80 kDa
GenBank Accession NumberBC107493
Gene Symbol ECM2
Gene ID (NCBI) 1842
RRIDAB_2878850
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94769
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for ECM2 antibody 21376-1-APDownload protocol
IF protocol for ECM2 antibody 21376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Neuropsychiatry

Cell Type-Specific Effects of Mutant DISC1: A Proteomics Study.

Authors - Meng Xia
View Article
humanIHC

Int J Biochem Cell Biol

ECM2, a prognostic biomarker for lower grade glioma, serves as a potential novel target for immunotherapy

Authors - Xingbo Cheng
View Article