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ECSIT Polyclonal antibody

ECSIT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27548-1-AP

Print datasheet

Synonyms

SITPEC, Protein SITPEC, ECSIT homolog (Drosophila)

验证数据展示

  • IHC staining of human ovary cancer using 27548-1-AP

    IHC staining of human ovary cancer using 27548-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27548-1-AP (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 27548-1-AP

    IHC staining of human ovary cancer using 27548-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27548-1-AP (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 27548-1-AP

    WB analysis using 27548-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27548-1-AP (ECSIT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells, mouse kidney tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27548-1-AP targets ECSIT in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ECSIT fusion protein Ag26877 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ECSIT homolog (Drosophila)
Synonyms SITPEC, Protein SITPEC, ECSIT homolog (Drosophila)
Calculated Molecular Weight 49 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberBC008279
Gene Symbol ECSIT
Gene ID (NCBI) 51295
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQ95
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ECSIT is a cytosolic adaptor protein associated with the toll-like receptor pathway. It has a distinct N-terminal mitochondrial targeting sequence, pentatricopeptide repeat motif, and a C-terminal pleckstrin homology domain. ECSIT regulates many biological processes like embryonic development, inflammation, cardiac function, and assembly of mitochondrial complex I. In macrophages, the activation of TLRs by inflammatory signals promotes the translocation of TRAF6 to mitochondria where it engages ECSIT to stimulate the production of mitochondrial reactive oxygen species (ROS).

实验方案

Product Specific Protocols
WB protocol for ECSIT antibody 27548-1-APDownload protocol
IHC protocol for ECSIT antibody 27548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols