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EDA Polyclonal antibody

EDA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25892-1-AP

Print datasheet

Synonyms

Ectodermal dysplasia protein, ectodysplasin A, ED1, ED1 A1, ED1 A2, EDA, EDA protein, EDA1, EDA2, HED, XHED, XLHED

验证数据展示

  • IHC staining of human liver using 25892-1-AP

    IHC staining of human liver using 25892-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25892-1-AP (EDA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse liver using 25892-1-AP

    WB analysis of mouse liver using 25892-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25892-1-AP (EDA Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25892-1-AP is EDA Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen EDA fusion protein Ag23154 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ectodysplasin A
Synonyms Ectodermal dysplasia protein, ectodysplasin A, ED1, ED1 A1, ED1 A2, EDA, EDA protein, EDA1, EDA2, HED, XHED, XLHED
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC126143
Gene Symbol EDA
Gene ID (NCBI) 1896
RRIDAB_2880286
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92838
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EDA antibody 25892-1-APDownload protocol
IHC protocol for EDA antibody 25892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Heliyon

Identification of cancer-associated fibroblast-related Ectodysplasin-A as a novel indicator for prognosis and immune response in gastric cancer

Authors - Ya Zhang
View Article
humanWB

Clin Cosmet Investig Dermatol

A Novel Ectodysplasin a Gene mutation of X-Linked Hypohidrotic Ectodermal Dysplasia

Authors - Yuan Zhuang
View Article