验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells, mouse liver, rat liver |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
26226-1-AP targets EDEM1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | EDEM1 fusion protein Ag24046 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ER degradation enhancer, mannosidase alpha-like 1 |
| 别名 | EDEM, EDEM1, KIAA0212 |
| 计算分子量 | 74 kDa |
| 观测分子量 | 66-75 kDa |
| GenBank蛋白编号 | BC019088 |
| 基因名称 | EDEM1 |
| Gene ID (NCBI) | 9695 |
| RRID | AB_2880433 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92611 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EDEM1 antibody 26226-1-AP | Download protocol |
| IHC protocol for EDEM1 antibody 26226-1-AP | Download protocol |
| IP protocol for EDEM1 antibody 26226-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene Small extracellular vesicles containing miR-30a-3p attenuate the migration and invasion of hepatocellular carcinoma by targeting SNAP23 gene. | ||
Front Mol Biosci Glutamate-Cysteine Ligase Catalytic Subunit Attenuated Hepatitis C Virus-Related Liver Fibrosis and Suppressed Endoplasmic Reticulum Stress. | ||
J Cell Biochem Erdj3 has an essential role for Z variant Alpha-1-Antitrypsin Degradation. | ||
bioRxiv A new role for IFRD1 in regulation of ER stress in bladder epithelial homeostasis | ||