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验证数据展示

  • WB analysis of 3T3-L1 using 27310-1-AP

    WB analysis of 3T3-L1 using 27310-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27310-1-AP (EDEM3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 27310-1-AP

    IP experiment of HEK-293 using 27310-1-AP

    IP result of anti-EDEM3 (IP:27310-1-AP, 4ug; Detection:27310-1-AP 1:300) with HEK-293 cells lysate 2600ug.

  • IHC staining of human stomach cancer using 27310-1-AP

    IHC staining of human stomach cancer using 27310-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27310-1-AP (EDEM3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EDEM3 Polyclonal antibody

EDEM3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 27310-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA

别名

ER degradation-enhancing alpha-mannosidase-like protein 3, EC:3.2.1.113, C1orf22, Alpha-1,2-mannosidase EDEM3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected in3T3-L1 cells, HEK-293 cell
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27310-1-AP targets EDEM3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 EDEM3 fusion protein Ag26123 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ER degradation enhancer, mannosidase alpha-like 3
别名 ER degradation-enhancing alpha-mannosidase-like protein 3, EC:3.2.1.113, C1orf22, Alpha-1,2-mannosidase EDEM3
计算分子量 105 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC016464
基因名称 EDEM3
Gene ID (NCBI) 80267
RRIDAB_2880839
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BZQ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EDEM3 antibody 27310-1-APDownload protocol
IHC protocol for EDEM3 antibody 27310-1-APDownload protocol
IP protocol for EDEM3 antibody 27310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols