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EDF1 Polyclonal antibody

EDF1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12419-1-AP

Print datasheet

Synonyms

EDF 1, EDF1, MBF1, Multiprotein bridging factor 1



经过测试的应用

Positive WB detected inJurkat cells, A549 cells, mouse pancreas tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EDF1 Ready-To-Use IHC Kit. EDF1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12419-1-AP targets EDF1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen EDF1 fusion protein Ag3063 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name endothelial differentiation-related factor 1
Synonyms EDF 1, EDF1, MBF1, Multiprotein bridging factor 1
Calculated Molecular Weight 148 aa, 16 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC015500
Gene Symbol EDF1
Gene ID (NCBI) 8721
RRIDAB_2230895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60869
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EDF1 antibody 12419-1-APDownload protocol
IHC protocol for EDF1 antibody 12419-1-APDownload protocol
IP protocol for EDF1 antibody 12419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP

Nat Commun

Long noncoding RNA licensing of obesity-linked hepatic lipogenesis and NAFLD pathogenesis.

Authors - Xu-Yun Zhao
humanWB

Front Neurol

Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.

Authors - Matthias Höllerhage
mouseWB

bioRxiv

Genetic deficiency of ribosomal rescue factor HBS1L causes retinal dystrophy associated with Pelota and EDF1 depletion

Authors - Shiyu Luo
mouseWB

Dis Model Mech

HBS1L deficiency causes retinal dystrophy in a child and a mouse model associated with defective development of photoreceptor cells

Authors - Shiyu Luo