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- KD/KO Validated
EDF1 Polyclonal antibody
EDF1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, A549 cells, mouse pancreas tissue |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
IP | See 1 publications below |
产品信息
12419-1-AP targets EDF1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | EDF1 fusion protein Ag3063 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | endothelial differentiation-related factor 1 |
Synonyms | EDF 1, EDF1, MBF1, Multiprotein bridging factor 1 |
Calculated Molecular Weight | 148 aa, 16 kDa |
Observed Molecular Weight | 18-20 kDa |
GenBank Accession Number | BC015500 |
Gene Symbol | EDF1 |
Gene ID (NCBI) | 8721 |
RRID | AB_2230895 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O60869 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for EDF1 antibody 12419-1-AP | Download protocol |
IHC protocol for EDF1 antibody 12419-1-AP | Download protocol |
IP protocol for EDF1 antibody 12419-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Long noncoding RNA licensing of obesity-linked hepatic lipogenesis and NAFLD pathogenesis. | ||
Front Neurol Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein. | ||
bioRxiv Genetic deficiency of ribosomal rescue factor HBS1L causes retinal dystrophy associated with Pelota and EDF1 depletion | ||
Dis Model Mech HBS1L deficiency causes retinal dystrophy in a child and a mouse model associated with defective development of photoreceptor cells |