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EEA1 Polyclonal antibody
EEA1 Polyclonal Antibody for WB, IF/ICC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat and More (3)
Applications
WB, IF/ICC, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HepG2 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, C6 cells |
| Positive IHC detected in | human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 5 publications below |
产品信息
28347-1-AP targets EEA1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | WB, IF/ICC, IHC, ELISA Application Description |
| Cited Applications | IF |
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse, monkey |
| Immunogen | EEA1 fusion protein Ag28814 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | early endosome antigen 1 |
| Synonyms | Cell and organelle markers, early endosome antigen 1, EEA1, Endosome Marker, MST105, MSTP105, ZFYVE2 |
| Calculated Molecular Weight | 162 kDa |
| Observed Molecular Weight | 170 kDa |
| GenBank Accession Number | NM_003566 |
| Gene Symbol | EEA1 |
| Gene ID (NCBI) | 8411 |
| RRID | AB_2881117 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15075 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EEA1 antibody 28347-1-AP | Download protocol |
| IHC protocol for EEA1 antibody 28347-1-AP | Download protocol |
| IF protocol for EEA1 antibody 28347-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Nucleosomes enter cells by clathrin- and caveolin-dependent endocytosis. | ||
Front Immunol Host MKRN1-Mediated Mycobacterial PPE Protein Ubiquitination Suppresses Innate Immune Response. | ||
Chemistry Investigation of Glycosylphosphatidylinositol (GPI)-Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction | ||
Toxicol Lett Anti-ricin toxin human neutralizing antibodies and DMAbs protection against ricin toxin poisoning |
