验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HepG2 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, C6 cells |
| Positive IHC detected in | human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 7 publications below |
产品信息
28347-1-AP targets EEA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | Human, Mouse, Rat |
| 文献引用反应性 | human, mouse, monkey |
| 免疫原 | EEA1 fusion protein Ag28814 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | early endosome antigen 1 |
| 别名 | Cell and organelle markers, early endosome antigen 1, EEA1, Endosome Marker, MST105, MSTP105, ZFYVE2 |
| 计算分子量 | 162 kDa |
| 观测分子量 | 170 kDa |
| GenBank蛋白编号 | NM_003566 |
| 基因名称 | EEA1 |
| Gene ID (NCBI) | 8411 |
| RRID | AB_2881117 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15075 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EEA1 antibody 28347-1-AP | Download protocol |
| IHC protocol for EEA1 antibody 28347-1-AP | Download protocol |
| IF protocol for EEA1 antibody 28347-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Nucleosomes enter cells by clathrin- and caveolin-dependent endocytosis. | ||
Front Immunol Host MKRN1-Mediated Mycobacterial PPE Protein Ubiquitination Suppresses Innate Immune Response. | ||
Chemistry Investigation of Glycosylphosphatidylinositol (GPI)-Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction | ||
Toxicol Lett Anti-ricin toxin human neutralizing antibodies and DMAbs protection against ricin toxin poisoning | ||
Nat Commun Sortilin-mediated translocation of mitochondrial ACSL1 impairs adipocyte thermogenesis and energy expenditure in male mice |
