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  • Featured Product
  • KD/KO Validated

EEA1 Monoclonal antibody, PBS Only

EEA1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E11E4

Cat no : 68065-1-PBS

Print datasheet

Synonyms

ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1

验证数据展示

  • WB analysis of HepG2 using 68065-1-Ig (same clone as 68065-1-PBS)

    WB analysis of HepG2 using 68065-1-Ig (same clone as 68065-1-PBS)

    WB result of EEA1 antibody (68065-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEA1 transfected HepG2 cells. This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68065-1-Ig (same clone as 68065-1-PBS)

    IF Staining of HepG2 using 68065-1-Ig (same clone as 68065-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EEA1 antibody (68065-1-Ig, Clone: 1E11E4 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

  • WB analysis using 68065-1-Ig (same clone as 68065-1-PBS)

    WB analysis using 68065-1-Ig (same clone as 68065-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68065-1-Ig (EEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

产品信息

68065-1-PBS targets EEA1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen EEA1 fusion protein Ag28814 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name early endosome antigen 1
Synonyms ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1
Calculated Molecular Weight 162 kDa
Observed Molecular Weight 162 kDa
GenBank Accession NumberNM_003566
Gene Symbol EEA1
Gene ID (NCBI) 8411
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15075
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.