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- KD/KO Validated
EEF1A2 Polyclonal antibody
EEF1A2 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MCF-7 cells, mouse brain tissue, mouse heart tissue, mouse skeletal muscle tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy EEF1A2 Ready-To-Use IHC Kit. EEF1A2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16091-1-AP targets EEF1A2 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, RIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish, oyster |
| Immunogen | EEF1A2 fusion protein Ag6875 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | eukaryotic translation elongation factor 1 alpha 2 |
| Synonyms | Statin S1, Eukaryotic elongation factor 1 A-2, Elongation factor 1-alpha 2, EF-1-alpha-2, EEF1AL |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 50-53 kDa |
| GenBank Accession Number | BC000432 |
| Gene Symbol | EEF1A2 |
| Gene ID (NCBI) | 1917 |
| RRID | AB_2096979 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q05639 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EEF1A2, also named as EEF1AL, STN and Statin-S1, belongs to the GTP-binding elongation factor family and EF-Tu/EF-1A subfamily. It promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 may be critical in the development of ovarian cancer. The MW of EEF1A2 is 50-53kd. This antibody can recognize both EEF1A1 and EEF1A2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EEF1A2 antibody 16091-1-AP | Download protocol |
| IHC protocol for EEF1A2 antibody 16091-1-AP | Download protocol |
| IP protocol for EEF1A2 antibody 16091-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Oncol SNX16 activates c-Myc signaling by inhibiting ubiquitin-mediated proteasomal degradation of eEF1A2 in colorectal cancer development. | ||
Pharmacol Res Nannocystin ax, an eEF1A inhibitor, induces G1 cell cycle arrest and caspase-independent apoptosis through cyclin D1 downregulation in colon cancer in vivo. | ||
Sci Rep Biallelic mutations in the gene encoding eEF1A2 cause seizures and sudden death in F0 mice.
| ||
Br J Cancer Dissecting the expression of EEF1A1/2 genes in human prostate cancer cells: the potential of EEF1A2 as a hallmark for prostate transformation and progression. | ||
Hum Mol Genet Recapitulation of the EEF1A2 D252H neurodevelopmental disorder-causing missense mutation in mice reveals a toxic gain of function. |