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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 66806-1-Ig (same clone as 66806-1-PBS)

    WB analysis using 66806-1-Ig (same clone as 66806-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 66806-1-Ig (same clone as 66806-1-PBS)

    WB analysis of mouse brain using 66806-1-Ig (same clone as 66806-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

  • WB analysis of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

    WB analysis of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

    IHC staining of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66806-1-Ig (EEF1A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1A2 antibody included
  • IHC staining of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

    IHC staining of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66806-1-Ig (EEF1A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1A2 antibody included

EEF1A2 Monoclonal antibody, PBS Only

EEF1A2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66806-1-PBS

产品说明书

CloneNo. 4F3F4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, Indirect ELISA

别名

Eukaryotic elongation factor 1 A-2, Elongation factor 1-alpha 2, EF-1-alpha-2, EEF1AL, eEF1A-2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EEF1A2 Ready-To-Use IHC Kit. EEF1A2 primary antibody included.

产品信息

66806-1-PBS targets EEF1A2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 EEF1A2 fusion protein Ag6875 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation elongation factor 1 alpha 2
别名 Eukaryotic elongation factor 1 A-2, Elongation factor 1-alpha 2, EF-1-alpha-2, EEF1AL, eEF1A-2
计算分子量 50 kDa
观测分子量 48-50 kDa
GenBank蛋白编号BC000432
基因名称 EEF1A2
Gene ID (NCBI) 1917
RRIDAB_2882149
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ05639
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

eEF1A2 is one of two isoforms (eEF1A1 and eEF1A2) of eukaryotic elongation factor 1 alpha (eEF1A or eEF1α). The two isoforms share more than 90% sequence identity and have the similar function that being a protein translation factor involved in protein synthesis. Addition, eEF1A2 plays an important role in cell cycle regulation, heat-shock response, aging, posttranslational modifications, and signal transduction (PMID: 25905039). eEF1A2 is expressed in heart, brain tissue, diaphragm and skeletal muscle while eEF1A1 is expressed ubiquitously (PMID:14588074). And the eEF1A2 protein stimulates the phospholipid signaling and activates the Akt-dependent cell migration and actin remodeling that ultimately favors tumorigenesis. It is reported that eEF1A2 was related with kinds of cancers such as ovarian cancer, prostate cancer, pancreatic cancer, breast cancer and lung cancer (PMID:14588074; 24853801; 25744894; 25905039). An 48 kDa band has also been reported(PMID: 24285179).