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EEF1A2 Monoclonal antibody, PBS Only

EEF1A2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F3F4

Cat no : 66806-1-PBS

Print datasheet

Synonyms

Eukaryotic elongation factor 1 A-2, Elongation factor 1-alpha 2, EF-1-alpha-2, EEF1AL, eEF1A-2

验证数据展示

  • WB analysis using 66806-1-Ig (same clone as 66806-1-PBS)

    WB analysis using 66806-1-Ig (same clone as 66806-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 66806-1-Ig (same clone as 66806-1-PBS)

    WB analysis of mouse brain using 66806-1-Ig (same clone as 66806-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

  • WB analysis of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

    WB analysis of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

    IHC staining of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66806-1-Ig (EEF1A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1A2 antibody included
  • IHC staining of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

    IHC staining of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66806-1-Ig (EEF1A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1A2 antibody included
Planning an IHC experiment? We recommend our IHCeasy EEF1A2 Ready-To-Use IHC Kit. EEF1A2 primary antibody included.

产品信息

66806-1-PBS targets EEF1A2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen EEF1A2 fusion protein Ag6875 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation elongation factor 1 alpha 2
Synonyms Eukaryotic elongation factor 1 A-2, Elongation factor 1-alpha 2, EF-1-alpha-2, EEF1AL, eEF1A-2
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 48-50 kDa
GenBank Accession NumberBC000432
Gene Symbol EEF1A2
Gene ID (NCBI) 1917
RRIDAB_2882149
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ05639
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

eEF1A2 is one of two isoforms (eEF1A1 and eEF1A2) of eukaryotic elongation factor 1 alpha (eEF1A or eEF1α). The two isoforms share more than 90% sequence identity and have the similar function that being a protein translation factor involved in protein synthesis. Addition, eEF1A2 plays an important role in cell cycle regulation, heat-shock response, aging, posttranslational modifications, and signal transduction (PMID: 25905039). eEF1A2 is expressed in heart, brain tissue, diaphragm and skeletal muscle while eEF1A1 is expressed ubiquitously (PMID:14588074). And the eEF1A2 protein stimulates the phospholipid signaling and activates the Akt-dependent cell migration and actin remodeling that ultimately favors tumorigenesis. It is reported that eEF1A2 was related with kinds of cancers such as ovarian cancer, prostate cancer, pancreatic cancer, breast cancer and lung cancer (PMID:14588074; 24853801; 25744894; 25905039). An 48 kDa band has also been reported(PMID: 24285179).