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  • KD/KO Validated

EEF1B2 Polyclonal antibody

EEF1B2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10095-2-AP

Print datasheet

Synonyms

EEF1B, EEF1B1, EEF1B2, EF 1 beta, EF1B, Elongation factor 1 beta



经过测试的应用

Positive WB detected inPC-3 cells, RAW264.7, Jurkat cells, HEK-293 cells, HeLa cells, SKOV-3 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon tissue, human breast cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy EEF1B2 Ready-To-Use IHC Kit. EEF1B2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10095-2-AP targets EEF1B2 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, IP, RIP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen EEF1B2 fusion protein Ag0135 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation elongation factor 1 beta 2
Synonyms EEF1B, EEF1B1, EEF1B2, EF 1 beta, EF1B, Elongation factor 1 beta
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC000211
Gene Symbol EEF1B2
Gene ID (NCBI) 1933
RRIDAB_2096987
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24534
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human EEF1B2.

实验方案

Product Specific Protocols
WB protocol for EEF1B2 antibody 10095-2-APDownload protocol
IHC protocol for EEF1B2 antibody 10095-2-APDownload protocol
IF protocol for EEF1B2 antibody 10095-2-APDownload protocol
IP protocol for EEF1B2 antibody 10095-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
RIP

Nat Cell Biol

The MBNL3 splicing factor promotes hepatocellular carcinoma by increasing PXN expression through the alternative splicing of lncRNA-PXN-AS1.

Authors - Ji-Hang Yuan
humanWB,IF,IP

Mol Cell Biol

Translational repression by RNA-binding protein TIAR.

Authors - Mazan-Mamczarz Krystyna K
humanWB

Front Microbiol

Strain-Specific Contribution of Eukaryotic Elongation Factor 1 Gamma to the Translation of Influenza A Virus Proteins.

Authors - Shuhei Sammaibashi
mouse, humanWB, IHC, IF

PLoS One

Characterisation of Translation Elongation Factor eEF1B Subunit Expression in Mammalian Cells and Tissues and Co-Localisation with eEF1A2.

Authors - Yuan Cao
  • KD Validated
humanWB

Biotechniques

En masse analysis of nascent translation using microarrays.

Authors - Mazan-Mamczarz Krystyna K