• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 60329-1-Ig

    WB analysis using 60329-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of HeLa using 60329-1-Ig

    WB analysis of HeLa using 60329-1-Ig

    WB result of EEF1B2 antibody (60329-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1B2 transfected HeLa cells.

  • IF Staining of HeLa using 60329-1-Ig

    IF Staining of HeLa using 60329-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 60329-1-Ig(EEF1B2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human colon using 60329-1-Ig

    IHC staining of human colon using 60329-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60329-1-Ig (EEF1B2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EEF1B2 antibody included
  • Featured Product
  • KD/KO验证

EEF1B2 Monoclonal antibody

EEF1B2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60329-1-Ig

产品说明书

CloneNo. 3E1C11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EF-1-beta, EF1B, EF 1 beta, EEF1B1, EEF1B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EEF1B2 Ready-To-Use IHC Kit. EEF1B2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60329-1-Ig targets EEF1B2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EEF1B2 fusion protein Ag0135 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation elongation factor 1 beta 2
别名 EF-1-beta, EF1B, EF 1 beta, EEF1B1, EEF1B
计算分子量 25 kDa
观测分子量 30-34 kDa
GenBank蛋白编号BC000211
基因名称 EEF1B2
Gene ID (NCBI) 1933
RRIDAB_2881438
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP24534
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE.

实验方案

Product Specific Protocols
WB protocol for EEF1B2 antibody 60329-1-IgDownload protocol
IHC protocol for EEF1B2 antibody 60329-1-IgDownload protocol
IF protocol for EEF1B2 antibody 60329-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols