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  • Featured Product
  • KD/KO Validated

EEF1D Monoclonal antibody, PBS Only

EEF1D Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3B1B11

Cat no : 60085-1-PBS

Print datasheet

Synonyms

EF-1-delta, EF1D, EF 1D, EF 1 delta, Antigen NY-CO-4

验证数据展示

  • WB analysis of Jurkat using 60085-1-Ig (same clone as 60085-1-PBS)

    WB analysis of Jurkat using 60085-1-Ig (same clone as 60085-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 60085-1-Ig (same clone as 60085-1-PBS)

    IP experiment of MCF-7 using 60085-1-Ig (same clone as 60085-1-PBS)

    IP result of anti-EEF1D (IP:60085-1-Ig, 5ug; Detection:60085-1-Ig 1:1000) with MCF-7 cells lysate 3200ug. This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 60085-1-Ig (same clone as 60085-1-PBS)

    IHC staining of human pancreas cancer using 60085-1-Ig (same clone as 60085-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60085-1-Ig (EEF1D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1D antibody included
  • IF Staining of HeLa using 60085-1-Ig (same clone as 60085-1-PBS)

    IF Staining of HeLa using 60085-1-Ig (same clone as 60085-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1D antibody (60085-1-Ig, Clone: 3B1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy EEF1D Ready-To-Use IHC Kit. EEF1D primary antibody included.

产品信息

60085-1-PBS targets EEF1D in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen EEF1D fusion protein Ag0983 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Synonyms EF-1-delta, EF1D, EF 1D, EF 1 delta, Antigen NY-CO-4
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC007847
Gene Symbol EEF1D
Gene ID (NCBI) 1936
RRIDAB_2096996
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29692
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )