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验证数据展示

  • WB analysis using 68148-1-Ig (same clone as 68148-1-PBS)

    WB analysis using 68148-1-Ig (same clone as 68148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

  • WB analysis using 68148-1-Ig (same clone as 68148-1-PBS)

    WB analysis using 68148-1-Ig (same clone as 68148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68148-1-Ig (same clone as 68148-1-PBS)

    IF Staining of HeLa using 68148-1-Ig (same clone as 68148-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68148-1-Ig (same clone as 68148-1-PBS)

    IF Staining of HeLa using 68148-1-Ig (same clone as 68148-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

EEF1G Monoclonal antibody, PBS Only

EEF1G Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68148-1-PBS

产品说明书

CloneNo. 1G7C6

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Pig, Rabbit

应用

WB, IF/ICC, Indirect ELISA

别名

eEF 1B gamma, EEF1G, EF 1 gamma, EF1G, Elongation factor 1 gamma, GIG35

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68148-1-PBS targets EEF1G in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Pig, Rabbit samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Pig, Rabbit
免疫原 EEF1G fusion protein Ag0218 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation elongation factor 1 gamma
别名 eEF 1B gamma, EEF1G, EF 1 gamma, EF1G, Elongation factor 1 gamma, GIG35
计算分子量 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC006520
基因名称 EEF1G
Gene ID (NCBI) 1937
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP26641
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Eukaryotic translation elongation factor 1 (EEF1G, synonym: EF1G) is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.