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  • Featured Product
  • KD/KO Validated

EEF2 Polyclonal antibody, PBS Only

EEF2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20107-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 20107-1-AP (same clone as 20107-1-PBS)

    WB analysis using 20107-1-AP (same clone as 20107-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    FC experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human EEF2 (20107-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    WB analysis of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HeLa cells. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    IP experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    IP result of anti-EEF2 (IP:20107-1-AP, 3ug; Detection:20107-1-AP 1:500) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

    IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF2 antibody included
  • IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

    IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF2 antibody included
  • IHC staining of human colon using 20107-1-AP (same clone as 20107-1-PBS)

    IHC staining of human colon using 20107-1-AP (same clone as 20107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20107-1-AP (EEF2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF2 antibody included
  • IF Staining of HepG2 using 20107-1-AP (same clone as 20107-1-PBS)

    IF Staining of HepG2 using 20107-1-AP (same clone as 20107-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (20107-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy EEF2 Ready-To-Use IHC Kit. EEF2 primary antibody included.

产品信息

20107-1-PBS targets EEF2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen EEF2 fusion protein Ag13826 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation elongation factor 2
Synonyms
Calculated Molecular Weight 858 aa, 95 kDa
Observed Molecular Weight 95-100 kDa
GenBank Accession NumberBC126259
Gene Symbol EEF2
Gene ID (NCBI) 1938
RRIDAB_10950401
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13639
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C