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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 20107-1-AP (same clone as 20107-1-PBS)

    WB analysis using 20107-1-AP (same clone as 20107-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    FC experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human EEF2 (20107-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    WB analysis of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HeLa cells. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    IP experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    IP result of anti-EEF2 (IP:20107-1-AP, 3ug; Detection:20107-1-AP 1:500) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

    IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF2 antibody included
  • IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

    IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF2 antibody included
  • IHC staining of human colon using 20107-1-AP (same clone as 20107-1-PBS)

    IHC staining of human colon using 20107-1-AP (same clone as 20107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20107-1-AP (EEF2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF2 antibody included
  • IF Staining of HepG2 using 20107-1-AP (same clone as 20107-1-PBS)

    IF Staining of HepG2 using 20107-1-AP (same clone as 20107-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (20107-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

EEF2 Polyclonal antibody, PBS Only

EEF2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 20107-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EEF2 Ready-To-Use IHC Kit. EEF2 primary antibody included.

产品信息

20107-1-PBS targets EEF2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EEF2 fusion protein Ag13826 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation elongation factor 2
别名 Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-
计算分子量 858 aa, 95 kDa
观测分子量 95-100 kDa
GenBank蛋白编号BC126259
基因名称 EEF2
Gene ID (NCBI) 1938
RRIDAB_10950401
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13639
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.