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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 20107-1-AP (same clone as 20107-1-PBS)

    WB analysis using 20107-1-AP (same clone as 20107-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    FC experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human EEF2 (20107-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    WB analysis of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HeLa cells. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    IP experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    IP result of anti-EEF2 (IP:20107-1-AP, 3ug; Detection:20107-1-AP 1:500) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

    IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF2 antibody included
  • IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

    IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF2 antibody included
  • IHC staining of human colon using 20107-1-AP (same clone as 20107-1-PBS)

    IHC staining of human colon using 20107-1-AP (same clone as 20107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20107-1-AP (EEF2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF2 antibody included
  • IF Staining of HepG2 using 20107-1-AP (same clone as 20107-1-PBS)

    IF Staining of HepG2 using 20107-1-AP (same clone as 20107-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (20107-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EEF2 Polyclonal antibody, PBS Only

EEF2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 20107-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EEF2 Ready-To-Use IHC Kit. EEF2 primary antibody included.

产品信息

20107-1-PBS targets EEF2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen EEF2 fusion protein Ag13826 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation elongation factor 2
Synonyms Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-
Calculated Molecular Weight 858 aa, 95 kDa
Observed Molecular Weight 95-100 kDa
GenBank Accession NumberBC126259
Gene Symbol EEF2
Gene ID (NCBI) 1938
RRIDAB_10950401
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13639
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.