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验证数据展示

  • WB analysis of PC-3 using 13510-1-AP

    WB analysis of PC-3 using 13510-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 13510-1-AP

    IP experiment of PC-3 using 13510-1-AP

    IP result of anti-eEF2K (IP:13510-1-AP, 4ug; Detection:13510-1-AP 1:500) with PC-3 cells lysate 3200ug.

  • IF Staining of PC-3 using 13510-1-AP

    IF Staining of PC-3 using 13510-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13510-1-AP (EEF2K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

eEF2K Polyclonal antibody

eEF2K Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 13510-1-AP

产品说明书

引用文献(5)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, IP, ELISA and More (1)

别名

eEF-2K, eEF-2 kinase, eEF 2K, eEF 2 kinase, EC:2.7.11.20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, Jurkat cells, HEK-293 cells
Positive IP detected inPC-3 cells
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13510-1-AP targets eEF2K in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IP, CoIP
经测试反应性 human
文献引用反应性human, rat
免疫原 eEF2K fusion protein Ag4432 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic elongation factor-2 kinase
别名 eEF-2K, eEF-2 kinase, eEF 2K, eEF 2 kinase, EC:2.7.11.20
计算分子量 725 aa, 100 kDa
观测分子量 100 kDa
GenBank蛋白编号BC032665
基因名称 EEF2K
Gene ID (NCBI) 29904
RRIDAB_2277761
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00418
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for eEF2K antibody 13510-1-APDownload protocol
IF protocol for eEF2K antibody 13510-1-APDownload protocol
IP protocol for eEF2K antibody 13510-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Lett

eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells.

Authors - Cheng Ji
View Article
humanWB

Oncol Lett

AMPK decreases ERK1/2 activity and cancer cell sensitivity to nutrition deprivation by mediating a positive feedback loop involving eEF2K.

Authors - Shujuan Tong
View Article
ratWB,IP,CoIP

Cells

SIRT1 Protects the Heart from ER Stress-Induced Injury by Promoting eEF2K/eEF2-Dependent Autophagy.

Authors - Julie Pires Da Silva
  • KD Validated
View Article
humanWB

Med Oncol

A drug repurposing study identifies novel FOXM1 inhibitors with in vitro activity against breast cancer cells

Authors - Khaled A N Abusharkh
View Article

J Mol Med (Berl)

FOXM1 plays a role in autophagy by transcriptionally regulating Beclin-1 and LC3 genes in human triple-negative breast cancer cells.

Authors - Zuhal Hamurcu
View Article