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EEFSEC Polyclonal antibody

EEFSEC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10628-1-AP

Print datasheet

Synonyms

EEFSEC, EFSEC, Elongation factor sec, SELB

验证数据展示

  • WB analysis using 10628-1-AP

    WB analysis using 10628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 10628-1-AP

    IHC staining of human kidney using 10628-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, A431 cells, HEK-293 cells, Jurkat cells, HepG2 cells
Positive IHC detected inhuman kidney tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10628-1-AP targets EEFSEC in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen EEFSEC fusion protein Ag0980 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic elongation factor, selenocysteine-tRNA-specific
Synonyms EEFSEC, EFSEC, Elongation factor sec, SELB
Calculated Molecular Weight 63 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC007933
Gene Symbol EEFSEC
Gene ID (NCBI) 60678
RRIDAB_2293378
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57772
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The amino acid selenocysteine (Sec) is incorporated into a small group of proteins that are involved in preventing cellular oxidative stress. The Sec-specific elongation factor eEFec delivers the aminoacylated selenocysteine-tRNA (Sec-tRNASec) to the ribosome and suppresses UGA codons that are upstream of Sec Insertion Sequence (SECIS) elements bound by SECIS binding protein 2 (SBP2)[PMID:22992746]. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon.

实验方案

Product Specific Protocols
WB protocol for EEFSEC antibody 10628-1-APDownload protocol
IHC protocol for EEFSEC antibody 10628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Am J Physiol Cell Physiol

Daily protein supplementation attenuates immobilization-induced blunting of postabsorptive muscle mTORC1 activation in middle-aged men.

Authors - Nina Zeng
View Article