验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, Daudi cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15206-1-AP targets CRACR2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | CRACR2A fusion protein Ag7358 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | EF-hand calcium binding domain 4B |
| 别名 | EFCAB4B, RAB46, EF-hand calcium-binding domain-containing protein 4B, CRAC channel regulator 2A, Calcium release-activated channel regulator 2A |
| 计算分子量 | 46 kDa |
| 观测分子量 | 46 kDa, 80-90 kDa |
| GenBank蛋白编号 | BC004524 |
| 基因名称 | CRACR2A |
| Gene ID (NCBI) | 84766 |
| RRID | AB_2246413 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BSW2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CRACR2A antibody 15206-1-AP | Download protocol |
| IHC protocol for CRACR2A antibody 15206-1-AP | Download protocol |
| IF protocol for CRACR2A antibody 15206-1-AP | Download protocol |
| IP protocol for CRACR2A antibody 15206-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder. | ||
Sci Rep Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells. | ||
Biochem Biophys Res Commun A long variant of CRACR2A that belongs to the Rab GTPase protein family.
| ||
J Cell Biol Rab46 integrates Ca2+ and histamine signaling to regulate selective cargo release from Weibel-Palade bodies.
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