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EFCAB5 Polyclonal antibody

EFCAB5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24296-1-AP

Print datasheet

Synonyms

EF-hand calcium-binding domain-containing protein 5

验证数据展示

  • WB analysis of HepG2 using 24296-1-AP

    WB analysis of HepG2 using 24296-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24296-1-AP (EFCAB5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 24296-1-AP

    IHC staining of human liver cancer using 24296-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50.

  • IHC staining of human liver cancer using 24296-1-AP

    IHC staining of human liver cancer using 24296-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50.

  • IF Staining of HepG2 using 24296-1-AP

    IF Staining of HepG2 using 24296-1-AP

    Immunofluorescent analysis of HepG2 cells using 24296-1-AP (EFCAB5 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inhuman liver cancer tissue, human colon tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24296-1-AP targets EFCAB5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen EFCAB5 fusion protein Ag19274 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name EF-hand calcium binding domain 5
Synonyms EF-hand calcium-binding domain-containing protein 5
Calculated Molecular Weight 1503 aa, 173 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC137519
Gene Symbol EFCAB5
Gene ID (NCBI) 374786
RRIDAB_2879479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA4FU69
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EFCAB5 antibody 24296-1-APDownload protocol
IHC protocol for EFCAB5 antibody 24296-1-APDownload protocol
IF protocol for EFCAB5 antibody 24296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols