验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, K-562 cells, mouse kidney tissue, JAR cells, SW480 cells, human heart tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human heart tissue, human prostate cancer tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
12004-1-AP targets EFEMP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | EFEMP2 fusion protein Ag2640 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | EGF-containing fibulin-like extracellular matrix protein 2 |
| 别名 | Fibulin-4, Fibulin 4, FIBL-4, FIBL 4, FBLN4 |
| 计算分子量 | 443 aa, 49 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC010456 |
| 基因名称 | EFEMP2 |
| Gene ID (NCBI) | 30008 |
| RRID | AB_10667002 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95967 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EFEMP2, also known as Fibulin-4 is a 60 kDa extracellular matrix (ECM) glycoprotein that is a pivotal oncogene in tumor progression and metastasis. EFEMP2 is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EFEMP2 antibody 12004-1-AP | Download protocol |
| IHC protocol for EFEMP2 antibody 12004-1-AP | Download protocol |
| IP protocol for EFEMP2 antibody 12004-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Biol Ther STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2 | ||
Cell Mol Biol Lett EFEMP2 upregulates PD-L1 expression via EGFR/ERK1/2/c-Jun signaling to promote the invasion of ovarian cancer cells |
