EFEMP2 Polyclonal antibody
EFEMP2 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HT-29 cells, K-562 cells, mouse kidney tissue, JAR cells, SW480 cells, human heart tissue |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human heart tissue, human prostate cancer tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
12004-1-AP targets EFEMP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | EFEMP2 fusion protein Ag2640 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | EGF-containing fibulin-like extracellular matrix protein 2 |
Synonyms | Fibulin-4, Fibulin 4, FIBL-4, FIBL 4, FBLN4 |
Calculated Molecular Weight | 443 aa, 49 kDa |
Observed Molecular Weight | 60 kDa |
GenBank Accession Number | BC010456 |
Gene Symbol | EFEMP2 |
Gene ID (NCBI) | 30008 |
RRID | AB_10667002 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O95967 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for EFEMP2 antibody 12004-1-AP | Download protocol |
IHC protocol for EFEMP2 antibody 12004-1-AP | Download protocol |
IP protocol for EFEMP2 antibody 12004-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Biol Ther STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2 | ||
Cell Mol Biol Lett EFEMP2 upregulates PD-L1 expression via EGFR/ERK1/2/c-Jun signaling to promote the invasion of ovarian cancer cells |