• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MICU2 Polyclonal antibody

MICU2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20859-1-AP

Print datasheet

Synonyms

EFHA1, EF-hand domain-containing family member A1, Calcium uptake protein 2, mitochondrial

验证数据展示

  • WB analysis using 20859-1-AP

    WB analysis using 20859-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20859-1-AP (EFHA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 20859-1-AP

    IHC staining of human stomach cancer using 20859-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20859-1-AP (MICU2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, mouse spleen tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20859-1-AP targets MICU2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MICU2 fusion protein Ag14830 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name EF-hand domain family, member A1
Synonyms EFHA1, EF-hand domain-containing family member A1, Calcium uptake protein 2, mitochondrial
Calculated Molecular Weight 434 aa, 50 kDa
Observed Molecular Weight~40 kDa
GenBank Accession NumberBC034965
Gene Symbol MICU2
Gene ID (NCBI) 221154
RRIDAB_10695500
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYU8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MICU2 antibody 20859-1-APDownload protocol
IHC protocol for MICU2 antibody 20859-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols