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EFHD2 Recombinant antibody, PBS Only

EFHD2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240185H5

Cat no : 83264-5-PBS

Print datasheet

Synonyms

SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2, 240185H5

验证数据展示

  • WB analysis using 83264-5-RR (same clone as 83264-5-PBS)

    WB analysis using 83264-5-RR (same clone as 83264-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83264-5-RR (EFHD2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83264-5-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 83264-5-RR (same clone as 83264-5-PBS)

    IF Staining of A431 using 83264-5-RR (same clone as 83264-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using EFHD2 antibody (83264-5-RR, Clone: 240185H5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83264-5-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83264-5-RR (same clone as 83264-5-PBS)

    FC experiment of A431 using 83264-5-RR (same clone as 83264-5-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug EFHD2 Recombinant antibody (83264-5-RR, Clone:240185H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83264-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83264-5-RR

    Affinity and Kinetic Characterization of 83264-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83264-5-RR against Human EFHD2 were performed. The affinity constant is 1.42 nM.

Planning an IHC experiment? We recommend our IHCeasy EFHD2 Ready-To-Use IHC Kit. EFHD2 primary antibody included.

产品信息

The immunogen of 83264-5-PBS is EFHD2 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen EFHD2 fusion protein Ag35084 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name EF-hand domain family, member D2
Synonyms SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2, 240185H5
Calculated Molecular Weight27 kDa
GenBank Accession NumberBC023611
Gene Symbol EFHD2
Gene ID (NCBI) 79180
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96C19
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C