验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human brain tissue, A549 cells, rat brain tissue |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12480-1-AP targets Ephrin A3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | Ephrin A3 fusion protein Ag3188 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ephrin-A3 |
| 别名 | EFL 2, EFL2, EFNA3, Ehk1 L, EHK1 ligand, ephrin A3, EPLG3, LERK 3, LERK3 |
| 计算分子量 | 238 aa, 26 kDa |
| 观测分子量 | 26-35 kDa |
| GenBank蛋白编号 | BC017722 |
| 基因名称 | Ephrin A3 |
| Gene ID (NCBI) | 1944 |
| RRID | AB_10638305 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P52797 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Ephrin A3 antibody 12480-1-AP | Download protocol |
| IHC protocol for Ephrin A3 antibody 12480-1-AP | Download protocol |
| IP protocol for Ephrin A3 antibody 12480-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Research (Wash D C) Exercise-Induced miR-210 Promotes Cardiomyocyte Proliferation and Survival and Mediates Exercise-Induced Cardiac Protection against Ischemia/Reperfusion Injury | ||
Cancer Med EphrinA3 is a key regulator of malignant behaviors and a potential prognostic factor in lung adenocarcinoma. | ||
Biomed Res Int OSCC Exosomes Regulate miR-210-3p Targeting EFNA3 to Promote Oral Cancer Angiogenesis through the PI3K/AKT Pathway.
| ||
J Exp Clin Cancer Res The Ephrin tyrosine kinase a3 (EphA3) is a novel mediator of RAGE-prompted motility of breast cancer cells
| ||
Brain Res Bull Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats | ||
J Cell Mol Med microRNA-365 attenuated intervertebral disc degeneration through modulating nucleus pulposus cell apoptosis and extracellular matrix degradation by targeting EFNA3 |
