验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10208-1-AP targets EFTUD2 in WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, PLA |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | EFTUD2 fusion protein Ag0279 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | elongation factor Tu GTP binding domain containing 2 |
| 别名 | Snu114, SNRP116, KIAA0031, hSNU114, Elongation factor Tu GTP-binding domain-containing protein 2 |
| 计算分子量 | 116 kDa |
| 观测分子量 | 116 kDa |
| GenBank蛋白编号 | BC002360 |
| 基因名称 | EFTUD2 |
| Gene ID (NCBI) | 9343 |
| RRID | AB_2095834 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15029 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EFTUD2 encodes the 116 kDa U5 small nuclear ribonecleoprotein (snRNP) component, also named SNU114, it has GTP binding activity and is required for pre-mRNA splicing. Evolutionarily conserved human snRNP protein (U5-116kDa) is homologous to the ribosomal elongation factor EF-2 (ribosomal translocase). Defects in EFTUD2 are the cause of mandibulofacial dysostosis with microcephaly (MFDM). So far, acetylation and phosphorylation sites of EFTUD2 have been identified. Catolog#10208-1-AP is a rabbit polyclonal antibody raised against the N-terminal of human EFTUD2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EFTUD2 antibody 10208-1-AP | Download protocol |
| IF protocol for EFTUD2 antibody 10208-1-AP | Download protocol |
| IP protocol for EFTUD2 antibody 10208-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Immunol Syndromic immune disorder caused by a viable hypomorphic allele of spliceosome component Snrnp40. | ||
Nucleic Acids Res XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence. | ||
FASEB J Reduced prostaglandin I2 signaling in Arid5b-/- primary skeletal muscle cells attenuates myogenesis. | ||
Mol Cell Biol A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis. | ||
iScience Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells |
