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验证数据展示

  • WB analysis using 26661-1-AP

    WB analysis using 26661-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26661-1-AP (EGFL7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 26661-1-AP

    IHC staining of human placenta using 26661-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26661-1-AP (EGFL7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 26661-1-AP

    IHC staining of human placenta using 26661-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26661-1-AP (EGFL7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EGFL7 Polyclonal antibody

EGFL7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26661-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Multiple EGF-like domains protein 7, MEGF7, Epidermal growth factor-like protein 7, EGF-like protein 7, EGF like protein 7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, K-562 cells, RKO cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26661-1-AP targets EGFL7 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 EGFL7 fusion protein Ag24693 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 EGF-like-domain, multiple 7
别名 Multiple EGF-like domains protein 7, MEGF7, Epidermal growth factor-like protein 7, EGF-like protein 7, EGF like protein 7
计算分子量 273 aa, 30 kDa
观测分子量30 kDa
GenBank蛋白编号BC012377
基因名称 EGFL7
Gene ID (NCBI) 51162
RRIDAB_3669560
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHF1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epidermal growth factor-like protein 7 (EGFL7, also known as MEGF7, ZNEU1, and VE-STATIN) is a proangiogenic factor that has been widely described as having a vital role in tubulogenesis and regulation of angiogenesis, mainly during embryogenesis organogenesis (PMID: 22160377; 15162510). It also can reduce central nervous system inflammation and may play a role in tumorigenesis (PMID: 29483510; 37490307).

实验方案

Product Specific Protocols
WB protocol for EGFL7 antibody 26661-1-APDownload protocol
IHC protocol for EGFL7 antibody 26661-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols