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EGFL7 Polyclonal antibody

EGFL7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26661-1-AP

Print datasheet

Synonyms

Multiple EGF-like domains protein 7, MEGF7, Epidermal growth factor-like protein 7, EGF-like protein 7, EGF like protein 7

验证数据展示

  • WB analysis using 26661-1-AP

    WB analysis using 26661-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26661-1-AP (EGFL7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 26661-1-AP

    IHC staining of human placenta using 26661-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26661-1-AP (EGFL7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 26661-1-AP

    IHC staining of human placenta using 26661-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26661-1-AP (EGFL7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCOLO 320 cells, K-562 cells, RKO cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26661-1-AP targets EGFL7 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen EGFL7 fusion protein Ag24693 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name EGF-like-domain, multiple 7
Synonyms Multiple EGF-like domains protein 7, MEGF7, Epidermal growth factor-like protein 7, EGF-like protein 7, EGF like protein 7
Calculated Molecular Weight 273 aa, 30 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC012377
Gene Symbol EGFL7
Gene ID (NCBI) 51162
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHF1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EGFL7 antibody 26661-1-APDownload protocol
IHC protocol for EGFL7 antibody 26661-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols