Pikachurin Polyclonal antibody

Pikachurin Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14578-1-AP

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Synonyms

EGFLAM, EGF-like, fibronectin type-III and laminin G-like domain-containing protein, AGRNL, Agrin-like protein, AGRINL



经过测试的应用

Positive WB detected inhuman placenta tissue, PC-3 cells, SH-SY5Y cells
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14578-1-AP targets Pikachurin in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Pikachurin fusion protein Ag5986 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name EGF-like, fibronectin type III and laminin G domains
Synonyms EGFLAM, EGF-like, fibronectin type-III and laminin G-like domain-containing protein, AGRNL, Agrin-like protein, AGRINL
Calculated Molecular Weight 111 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC033177
Gene Symbol Pikachurin
Gene ID (NCBI) 133584
RRIDAB_2277653
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ63HQ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This antibody detects a 60-kDa band, which has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).

实验方案

Product Specific Protocols
WB protocol for Pikachurin antibody 14578-1-APDownload protocol
IF protocol for Pikachurin antibody 14578-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Stem Cells Dev

Pikachurin Is Partially Involved in the Synaptic Connection Between Donor and Host Cells in Late-Stage rd1 Mice Following Conspecific Photoreceptor Transplantation.

Authors - Chen Liang
humanWB,CoIP

Int Immunopharmacol

EGFLAM exhibits oncogenic activity and shows promise as a prognostic biomarker and therapeutic target in glioblastoma

Authors - Kaishu Li
mouseIF

iScience

The prion protein is required for normal responses to light stimuli by photoreceptors and bipolar cells

Authors - James F Striebel