验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells |
Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
Positive FC (Intra) detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83806-1-RR targets EGFR in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | human |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
别名 | Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R, 240603F10 |
计算分子量 | 134 kDa |
观测分子量 | 145-165 kDa |
GenBank蛋白编号 | NM_005228 |
基因名称 | EGFR |
Gene ID (NCBI) | 1956 |
RRID | AB_3671393 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P00533 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.
实验方案
Product Specific Protocols | |
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WB protocol for EGFR antibody 83806-1-RR | Download protocol |
IHC protocol for EGFR antibody 83806-1-RR | Download protocol |
IF protocol for EGFR antibody 83806-1-RR | Download protocol |
FC protocol for EGFR antibody 83806-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |