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- KD/KO Validated
CoraLite® Plus 488-conjugated EGFR Monoclonal antibody
EGFR Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2A2H10
验证数据展示
经过测试的应用
| Positive FC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66455-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66455 targets EGFR in FC (Intra) applications and shows reactivity with human samples.
| Tested Applications | FC (Intra) |
| Tested Reactivity | human |
| Immunogen | EGFR fusion protein Ag24947 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
| Synonyms | EGFR, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1 |
| Calculated Molecular Weight | 1210 aa, 134 kDa |
| Observed Molecular Weight | 145-165 kDa |
| GenBank Accession Number | BC094761 |
| Gene Symbol | EGFR |
| Gene ID (NCBI) | 1956 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P00533 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 EGFR antibody CL488-66455 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
