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验证数据展示

  • WB analysis of A431 using 51071-2-AP (same clone as 51071-2-PBS)

    WB analysis of A431 using 51071-2-AP (same clone as 51071-2-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 51071-2-AP (EGFR (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51071-2-PBS in a different storage buffer formulation.

  • IP experiment of A431 using 51071-2-AP (same clone as 51071-2-PBS)

    IP experiment of A431 using 51071-2-AP (same clone as 51071-2-PBS)

    IP result of anti-EGFR (C-terminal) (IP:51071-2-AP, 4ug; Detection:51071-2-AP 1:30000) with A431 cells lysate 1200 ug. This data was developed using the same antibody clone with 51071-2-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 51071-2-AP (same clone as 51071-2-PBS)

    IF Staining of A431 using 51071-2-AP (same clone as 51071-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR (C-terminal) antibody (51071-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51071-2-PBS in a different storage buffer formulation.

EGFR (C-terminal) Polyclonal antibody, PBS Only

EGFR (C-terminal) Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 51071-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.

产品信息

51071-2-PBS targets EGFR (C-terminal) in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
别名
计算分子量 1210 aa, 134 kDa
观测分子量 170 kDa
GenBank蛋白编号BC094761
基因名称 EGFR
Gene ID (NCBI) 1956
RRIDAB_2096449
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00533
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer. The antibody is specific to isoform1.