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PHD2/EGLN1 Polyclonal antibody

PHD2/EGLN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Cat no : 19886-1-AP

Print datasheet

Synonyms

EGLN1, PHD2, HIF-PH2, EGLN 1, ECYT3

验证数据展示

WB analysis using 19886-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 19886-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 19886-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PHD2/EGLN1 antibody included
IHC staining of human liver cancer using 19886-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PHD2/EGLN1 antibody included
IP experiment of HEK-293 using 19886-1-AP
IP result of anti-PHD2/EGLN1 (IP:19886-1-AP, 4ug; Detection:19886-1-AP 1:5000) with HEK-293 cells lysate 1280 ug.
IF Staining of A431 using 19886-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PHD2/EGLN1 antibody (19886-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Cat No. 19886-1-AP

Size

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经过测试的应用

Positive WB detected in	HCT 116 cells, mouse kidney tissue, rat heart tissue, HEK-293 cells, HEK-293T cells, HepG2 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Planning an IHC experiment? We recommend our IHCeasy PHD2/EGLN1 Ready-To-Use IHC Kit. PHD2/EGLN1 primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 14 publications below
IHC	See 2 publications below

产品信息

19886-1-AP targets PHD2/EGLN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	PHD2/EGLN1 fusion protein Ag13706 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	egl nine homolog 1 (C. elegans)
Synonyms	EGLN1, PHD2, HIF-PH2, EGLN 1, ECYT3
Calculated Molecular Weight	426 aa, 46 kDa
Observed Molecular Weight	46 kDa
GenBank Accession Number	BC005369
Gene Symbol	PHD2/EGLN1
Gene ID (NCBI)	54583
RRID	AB_10641986
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9GZT9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

EGLN1(Egl nine homolog 1) is also named as HIF-PH2,HPH-2,PHD2.It is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. EGLN1 plays a critical role in regulating HIF levels in EPO-producing cells in humans(PMID:16407130).Defects in EGLN1 are the cause of familial erythrocytosis type 3 (ECYT3).This antibody is specific to EGLN1.

实验方案

Product Specific Protocols
WB protocol for PHD2/EGLN1 antibody 19886-1-AP	Download protocol
IHC protocol for PHD2/EGLN1 antibody 19886-1-AP	Download protocol
IF protocol for PHD2/EGLN1 antibody 19886-1-AP	Download protocol
IP protocol for PHD2/EGLN1 antibody 19886-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation Authors - Yunzi Mao KO Validated View Article
	WB,IHC	Environ Int HIF-1α/m6A/NF-κB/CCL3 axis-mediated immunosurveillance participates in low level benzene-related erythrohematopoietic development toxicity Authors - Xiaowei Cong View Article
	WB	Cell Mol Life Sci Iron increases lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the HIF1α-PPARγ pathway. Authors - Chang-Chun Song View Article
human	WB	Int J Mol Sci Sustained Hypoxia Suppresses Joint Destruction in a Rat Model of Rheumatoid Arthritis via Negative Feedback of Hypoxia Inducible Factor-1α. Authors - Kenta Kaihara View Article
mouse	WB	Biomed Pharmacother A novel PHD2 inhibitor acteoside from Cistanche tubulosa induces skeletal muscle mitophagy to improve cancer-related fatigue. Authors - Shilei Zhang View Article
human	WB	Sci Rep Mitochondrial MUL1 E3 ubiquitin ligase regulates Hypoxia Inducible Factor (HIF-1α) and metabolic reprogramming by modulating the UBXN7 cofactor protein. Authors - Lucia Cilenti View Article

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PHD2/EGLN1 Polyclonal antibody

PHD2/EGLN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

验证数据展示

WB analysis using 19886-1-AP

WB analysis using 19886-1-AP

IHC staining of human liver cancer using 19886-1-AP

IHC staining of human liver cancer using 19886-1-AP

IP experiment of HEK-293 using 19886-1-AP

IF Staining of A431 using 19886-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

HIF-1α/m6A/NF-κB/CCL3 axis-mediated immunosurveillance participates in low level benzene-related erythrohematopoietic development toxicity

Iron increases lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the HIF1α-PPARγ pathway.

Sustained Hypoxia Suppresses Joint Destruction in a Rat Model of Rheumatoid Arthritis via Negative Feedback of Hypoxia Inducible Factor-1α.

A novel PHD2 inhibitor acteoside from Cistanche tubulosa induces skeletal muscle mitophagy to improve cancer-related fatigue.

Mitochondrial MUL1 E3 ubiquitin ligase regulates Hypoxia Inducible Factor (HIF-1α) and metabolic reprogramming by modulating the UBXN7 cofactor protein.