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验证数据展示

  • IF Staining of HepG2 using 83321-1-RR (same clone as 83321-1-PBS)

    IF Staining of HepG2 using 83321-1-RR (same clone as 83321-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EGLN3 antibody (83321-1-RR, Clone: 240165A6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83321-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83321-1-RR (same clone as 83321-1-PBS)

    FC experiment of MCF-7 using 83321-1-RR (same clone as 83321-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Egln3 Recombinant Antibody (83321-1-RR, Clone:240165A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83321-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83321-1-RR (same clone as 83321-1-PBS)

    FC experiment of A549 using 83321-1-RR (same clone as 83321-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Egln3 Recombinant Antibody (83321-1-RR, Clone:240165A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83321-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00347-1

    Cytometric bead array standard curve of MP00347-1

    Cytometric bead array standard curve of MP00347-1, EGLN3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83321-1-PBS. Detection antibody: 83321-4-PBS. Standard: Ag27464. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83321-1-PBS

    Affinity and Kinetic Characterization of 83321-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 83321-1-PBS against Human EGLN3 was performed. The affinity constant is 4.82 nM.

  • Affinity and Kinetic Characterization of 83321-1-RR

    Affinity and Kinetic Characterization of 83321-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83321-1-RR against Human EGLN3 were performed. The affinity constant is 1.82 nM.

EGLN3 Recombinant antibody, PBS Only (Capture)

EGLN3 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83321-1-PBS

产品说明书

CloneNo. 240165A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

PHD3, HIF prolyl hydroxylase 3, HIF PH3, Egl nine homolog 3, EC:1.14.11.29

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EGLN3 Ready-To-Use IHC Kit. EGLN3 primary antibody included.

产品信息

83321-1-PBS targets EGLN3 as part of a matched antibody pair:

MP00347-1: 83321-1-PBS capture and 83321-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 EGLN3 fusion protein Ag27464 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 egl nine homolog 3 (C. elegans)
别名 PHD3, HIF prolyl hydroxylase 3, HIF PH3, Egl nine homolog 3, EC:1.14.11.29
计算分子量 27 kDa
观测分子量27 kDa
GenBank蛋白编号BC010992
基因名称 EGLN3
Gene ID (NCBI) 112399
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H6Z9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.