验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, human peripheral blood platelets, rat brain tissue, mouse kidney tissue |
Positive IHC detected in | mouse lung tissue, human brain tissue, mouse brain tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25320-1-AP targets EHD3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IF, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, zebrafish |
免疫原 | EHD3 fusion protein Ag18216 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | EH-domain containing 3 |
别名 | EH domain containing 3, EH domain containing protein 3, EHD2, EHD3, PAST homolog 3, PAST3 |
计算分子量 | 535 aa, 61 kDa |
观测分子量 | 61 kDa |
GenBank蛋白编号 | BC156996 |
基因名称 | EHD3 |
Gene ID (NCBI) | 30845 |
RRID | AB_2880029 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9NZN3 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for EHD3 antibody 25320-1-AP | Download protocol |
IHC protocol for EHD3 antibody 25320-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.
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Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.
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EBioMedicine EHD1 impairs decidualization by regulating the Wnt4/β-catenin signaling pathway in recurrent implantation failure. | ||
EMBO J Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.
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Int J Mol Sci Proteomic Analysis of Dysfunctional Liver Sinusoidal Endothelial Cells Reveals Substantial Differences in Most Common Experimental Models of Chronic Liver Diseases | ||
Am J Physiol Renal Physiol Unique miRNome and transcriptome profiles underlie microvascular heterogeneity in mouse kidney |