验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human peripheral blood platelets, rat brain tissue, mouse kidney tissue |
| Positive IHC detected in | mouse lung tissue, human brain tissue, mouse brain tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25320-1-AP targets EHD3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Immunogen | EHD3 fusion protein Ag18216 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | EH-domain containing 3 |
| Synonyms | EH domain containing 3, EH domain containing protein 3, EHD2, EHD3, PAST homolog 3, PAST3 |
| Calculated Molecular Weight | 535 aa, 61 kDa |
| Observed Molecular Weight | 61 kDa |
| GenBank Accession Number | BC156996 |
| Gene Symbol | EHD3 |
| Gene ID (NCBI) | 30845 |
| RRID | AB_2880029 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZN3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EHD3 antibody 25320-1-AP | Download protocol |
| IHC protocol for EHD3 antibody 25320-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.
| ||
Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.
| ||
EBioMedicine EHD1 impairs decidualization by regulating the Wnt4/β-catenin signaling pathway in recurrent implantation failure. | ||
EMBO J Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.
| ||
Int J Mol Sci Proteomic Analysis of Dysfunctional Liver Sinusoidal Endothelial Cells Reveals Substantial Differences in Most Common Experimental Models of Chronic Liver Diseases | ||
Am J Physiol Renal Physiol Unique miRNome and transcriptome profiles underlie microvascular heterogeneity in mouse kidney |
