验证数据展示
经过测试的应用
Positive WB detected in | mouse kidney tissue, SMMC-7721 cells, HepG2 cells, mouse liver tissue, rat kidney tissue |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 6 publications below |
产品信息
26570-1-AP targets EHHADH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | mouse |
免疫原 | EHHADH fusion protein Ag24237 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase |
别名 | ECHD, EHHADH, L PBE, LBFP, LBP, PBE, PBFE |
计算分子量 | 723 aa, 79 kDa |
观测分子量 | 69 kDa |
GenBank蛋白编号 | BC038948 |
基因名称 | EHHADH |
Gene ID (NCBI) | 1962 |
RRID | AB_2880556 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q08426 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for EHHADH antibody 26570-1-AP | Download protocol |
IHC protocol for EHHADH antibody 26570-1-AP | Download protocol |
IF protocol for EHHADH antibody 26570-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation | ||
Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity | ||
Int J Mol Sci Ameliorative Effect of Ethanolic Echinacea purpurea against Hyperthyroidism-Induced Oxidative Stress via AMRK and PPAR Signal Pathway Using Transcriptomics and Network Pharmacology Analysis | ||
Int J Biochem Cell Biol Tamoxifen upregulates the peroxisomal β-oxidation enzyme Enoyl CoA hydratase and 3-hydroxyacyl CoA hydratase ameliorating hepatic lipid accumulation in mice | ||
Geroscience Coordinated transcriptional upregulation of oxidative metabolism proteins in long-lived endocrine mutant mice |