EHHADH Polyclonal antibody

EHHADH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26570-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

ECHD, EHHADH, L PBE, LBFP, LBP, PBE, PBFE

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse kidney tissue, SMMC-7721 cells, HepG2 cells, mouse liver tissue, rat kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 6 publications below

产品信息

26570-1-AP targets EHHADH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 EHHADH fusion protein Ag24237 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
别名 ECHD, EHHADH, L PBE, LBFP, LBP, PBE, PBFE
计算分子量 723 aa, 79 kDa
观测分子量 69 kDa
GenBank蛋白编号BC038948
基因名称 EHHADH
Gene ID (NCBI) 1962
RRIDAB_2880556
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ08426
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EHHADH antibody 26570-1-APDownload protocol
IHC protocol for EHHADH antibody 26570-1-APDownload protocol
IF protocol for EHHADH antibody 26570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao
mouseWB

Cell Metab

Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity

Authors - Zilong Zhang
mouseWB

Cell Rep

Feedback repression of PPARα signaling by Let-7 microRNA.

Authors - Tomoki Yagai
mouseWB

Int J Mol Sci

Ameliorative Effect of Ethanolic Echinacea purpurea against Hyperthyroidism-Induced Oxidative Stress via AMRK and PPAR Signal Pathway Using Transcriptomics and Network Pharmacology Analysis

Authors - Yingli Zhu
mouseWB

Int J Biochem Cell Biol

Tamoxifen upregulates the peroxisomal β-oxidation enzyme Enoyl CoA hydratase and 3-hydroxyacyl CoA hydratase ameliorating hepatic lipid accumulation in mice

Authors - Ziling Zhang
mouseWB

Geroscience

Coordinated transcriptional upregulation of oxidative metabolism proteins in long-lived endocrine mutant mice

Authors - Ahmed M Elmansi
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