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验证数据展示

  • IP experiment of K-562 using 55436-1-AP

    IP experiment of K-562 using 55436-1-AP

    IP result of anti-EHMT1 (IP:55436-1-AP, 4ug; Detection:55436-1-AP 1:500) with K-562 cells lysate 1360 ug.

  • IF Staining of HeLa using 55436-1-AP

    IF Staining of HeLa using 55436-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EHMT1 antibody (55436-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

EHMT1 Polyclonal antibody

EHMT1 Polyclonal Antibody for IF/ICC, IP, ELISA
Cat No. 55436-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IP, ELISA

别名

DEL9q34, EHMT1, Eu HMTase1, EUHMTASE1, FP13812, G9a like protein 1, GLP, GLP1, H3 K9 HMTase 5, KIAA1876, KMT1D, Lysine N methyltransferase 1D

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inK-562 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55436-1-AP targets EHMT1 in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 euchromatic histone-lysine N-methyltransferase 1
别名 DEL9q34, EHMT1, Eu HMTase1, EUHMTASE1, FP13812, G9a like protein 1, GLP, GLP1, H3 K9 HMTase 5, KIAA1876, KMT1D, Lysine N methyltransferase 1D
计算分子量 141 kDa
观测分子量150-170 kDa
GenBank蛋白编号NM_024757
基因名称 EHMT1
Gene ID (NCBI) 79813
RRIDAB_3670252
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H9B1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EHMT1, also known as GLP (G9a-like protein), encodes a histone lysine methyltransferase that controls brown adipose cell fate, adaptive thermogenesis, and glucose homeostasis in vivo (PMID: 24196706). EHMTs, along with LSD1 (an H3K4 demethylase), are components of the REST complex, which silences neuronal pathway genes in non-neuronal cells. EHMT1 is mainly responsible for the monomethylation and dimethylation of histone H3 lysine 9 (H3K9), forming a heteromeric complex with EHMT2 (G9a) in euchromatin (PMID: 34214254, 37663929).

实验方案

Product Specific Protocols
IF protocol for EHMT1 antibody 55436-1-APDownload protocol
IP protocol for EHMT1 antibody 55436-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols