验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IF/ICC detected in | A431 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1500-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 8 publications below |
产品信息
66689-1-Ig targets EHMT2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | EHMT2 fusion protein Ag27477 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | euchromatic histone-lysine N-methyltransferase 2 |
| 别名 | EHMT2/G9a, Euchromatic histone-lysine N-methyltransferase 2, EC:2.1.1.-, C6orf30, BAT8 |
| 计算分子量 | 1210 aa, 132 kDa |
| 观测分子量 | 150 kDa and 170 kDa |
| GenBank蛋白编号 | BC018718 |
| 基因名称 | EHMT2 |
| Gene ID (NCBI) | 10919 |
| RRID | AB_2882043 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q96KQ7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EHMT2 antibody 66689-1-Ig | Download protocol |
| IF protocol for EHMT2 antibody 66689-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration | ||
Nat Commun PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression. | ||
Am J Cancer Res HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma. | ||
iScience Linkage of methionine addiction, histone lysine hypermethylation, and malignancy. | ||
Front Cell Dev Biol Emerin interacts with histone methyltransferases to regulate repressive chromatin at the nuclear periphery |