验证数据展示
经过测试的应用
| Positive WB detected in | HT-1080 cells, U-937 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
11734-1-AP targets EID1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | EID1 fusion protein Ag2336 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | EP300 interacting inhibitor of differentiation 1 |
| 别名 | 21 kDa pRb-associated protein, C15orf3, CRI 1, CRI1, E1A-like inhibitor of differentiation 1 |
| 计算分子量 | 187 aa, 21 kDa |
| 观测分子量 | 20-30 kDa |
| GenBank蛋白编号 | BC024151 |
| 基因名称 | EID1 |
| Gene ID (NCBI) | 23741 |
| RRID | AB_10640804 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y6B2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EID1(EP300 interacting inhibitor of differentiation 1) is a member of the EID protein family, which regulates differentiation, transcription and acetyltransferase activity. EID1 function was originally identified as a transcriptional repressor which regulates cell cycle and differentiation, and leads to genome inactivation and instability by interacting with E3 ubiquitin ligase MDM2. EID1 is mainly located in the cytoplasm. It was translocated into the nuclei under stressed or pathological conditions such as AD patients and APP Tg mice brains.(PMID: 22186421, PMID: 31381951,PMID: 30926163).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EID1 antibody 11734-1-AP | Download protocol |
| IHC protocol for EID1 antibody 11734-1-AP | Download protocol |
| IF protocol for EID1 antibody 11734-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A A comprehensive method for detecting ubiquitinated substrates using TR-TUBE. | ||
Mol Cell Proteomics Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924. | ||
Folia Histochem Cytobiol EID1 plays a protective role in early-onset pre-eclampsia via promoting proliferation and invasion in trophoblast cells. | ||