验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, HeLa cells, HEK-293T cells, Jurkat cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
11649-2-AP targets EIF1AX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | EIF1AX fusion protein Ag2248 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 1A, X-linked |
| 别名 | eIF-1A X isoform, eIF1A X isoform, EIF1A, eIF 4C, eIF 1A X isoform |
| 计算分子量 | 16 kDa |
| 观测分子量 | 16 kDa |
| GenBank蛋白编号 | BC000793 |
| 基因名称 | EIF1AX |
| Gene ID (NCBI) | 1964 |
| RRID | AB_2097803 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P47813 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Eukaryotic translation initiation factor 1A, X-chromosome(EIF1AX), designated as to distinguish from the Y-linked homolog, has an unblocked N-terminal proline, which was consistent with the general pattern of eukaryotic protein. It's may be required for maximal rate of protein biosynthesis. Like EIF4C, it enhances ribosome dissociation into subunits and stabilizeds the binding of the initiator Met-tRNA(l) to 40S ribosomal subunits.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF1AX antibody 11649-2-AP | Download protocol |
| IHC protocol for EIF1AX antibody 11649-2-AP | Download protocol |
| IF protocol for EIF1AX antibody 11649-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Prolif Transcriptional repression of p21 by EIF1AX promotes the proliferation of breast cancer cells. | ||
Sci Adv Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses |
