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验证数据展示

  • WB analysis of MCF-7 using 11193-1-AP

    WB analysis of MCF-7 using 11193-1-AP

    WB result of EIF1AY antibody (11193-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF1AY transfected MCF-7 cells.

  • WB analysis of MCF-7 using 11193-1-AP

    WB analysis of MCF-7 using 11193-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11193-1-AP (EIF1AY antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 11193-1-AP

    IF Staining of MCF-7 using 11193-1-AP

    Immunofluorescent analysis of MCF-7 cells using 11193-1-AP (EIF1AY antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

EIF1AY Polyclonal antibody

EIF1AY Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 11193-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Eukaryotic translation initiation factor 1A, Y-chromosomal, eIF-4C, eIF-1A Y isoform, eIF1A Y isoform, eIF 4C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, MCF-7 cells, HEK-293 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11193-1-AP targets EIF1AY in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 EIF1AY fusion protein Ag1680 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 1A, Y-linked
别名 Eukaryotic translation initiation factor 1A, Y-chromosomal, eIF-4C, eIF-1A Y isoform, eIF1A Y isoform, eIF 4C
计算分子量 16 kDa
观测分子量 16-20 kDa
GenBank蛋白编号BC005248
基因名称 EIF1AY
Gene ID (NCBI) 9086
RRIDAB_2262159
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14602
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF1AY is 1 of 5 NRY genes that are expressed in many tissues and have homologs on the X chromosome, which is an essential translation initiation factor. EIF1AY seems to be required for maximal rate of protein biosynthesis, and enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. This is a rabbit polyclonal antibody raised against the full length of human EIF1AY, and can recognize both EIF1AY and EIF1AX.

实验方案

Product Specific Protocols
WB protocol for EIF1AY antibody 11193-1-APDownload protocol
IF protocol for EIF1AY antibody 11193-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols