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EIF1, EIF1B Polyclonal antibody

EIF1, EIF1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15887-1-AP

Print datasheet

Synonyms

EIF1B, GC20

验证数据展示

  • WB analysis of L02 using 15887-1-AP

    WB analysis of L02 using 15887-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15887-1-AP (EIF1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15887-1-AP

    IHC staining of human kidney using 15887-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15887-1-AP (EIF1B Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 15887-1-AP

    IHC staining of human kidney using 15887-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15887-1-AP (EIF1B Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inL02 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15887-1-AP targets EIF1, EIF1B in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen EIF1, EIF1B fusion protein Ag8673 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 1B
Synonyms EIF1B, GC20
Calculated Molecular Weight 113 aa, 13 kDa
Observed Molecular Weight14 kDa
GenBank Accession NumberBC006996
Gene Symbol EIF1B
Gene ID (NCBI) 10289
RRIDAB_2878196
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60739
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF1, EIF1B antibody 15887-1-APDownload protocol
IHC protocol for EIF1, EIF1B antibody 15887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols