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EIF2AK4 Polyclonal antibody

EIF2AK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27944-1-AP

Print datasheet

Synonyms

GCN2-like protein, GCN2 like protein, GCN2, Eukaryotic translation initiation factor 2-alpha kinase 4, eIF-2-alpha kinase GCN2

验证数据展示

  • IHC staining of human small intestine using 27944-1-AP

    IHC staining of human small intestine using 27944-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27944-1-AP (EIF2AK4 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human small intestine using 27944-1-AP

    IHC staining of human small intestine using 27944-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27944-1-AP (EIF2AK4 antibody) at dilution of 1:100 (under 10x lens).

  • WB analysis using 27944-1-AP

    WB analysis using 27944-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27944-1-AP (EIF2AK4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, TT cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27944-1-AP targets EIF2AK4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen EIF2AK4 fusion protein Ag27617 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2 alpha kinase 4
Synonyms GCN2-like protein, GCN2 like protein, GCN2, Eukaryotic translation initiation factor 2-alpha kinase 4, eIF-2-alpha kinase GCN2
Calculated Molecular Weight 187 kDa
Observed Molecular Weight~200 kDa
GenBank Accession NumberBC009350
Gene Symbol EIF2AK4
Gene ID (NCBI) 440275
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2K8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF2AK4 antibody 27944-1-APDownload protocol
IHC protocol for EIF2AK4 antibody 27944-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols